New method for determination of ten pesticides in human blood

An analytical method was developed for precise identification and quantitat ion of 10 pesticides in human brood. The pesticides studied, which have app eared frequently in actual cases, were endosulfan, lindane, parathion, ethy l-azinphos, diazinon, malathion, alachlor, tetradifon, fenthion and dicofol (o-p ' and p-p ' isomers). The current method replaces an earlier method w hich involved liquid-liquid extraction with a mixture of n-hexane-benzene ( 1 + 1). The extraction is performed by solid-phase extraction, with C-18 ca rtridges and 2 internal standards, perthane and triphenylphosphate. Eluates were analyzed by gas chromatography (GC) with nitrogen-phosphorus and elec trochemical detectors. Results were confirmed by GC-mass spectrometry in th e electron impact mode. Blood blank samples spiked with 2 standard mixtures and an internal standard were used for quantitation. Mean recoveries range d from 71.83 to 97.10%. Detection and quantitation limits are reported for each pesticide. Examples are provided to show the application of the presen t method to actual samples.