Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes

The Bacillus subtilis lacA gene, coding for beta -galactosidase, has been e xplored as a new site able to accept DNA sequences from nonreplicating deli very vectors. Two such delivery expression vectors have been constructed an d shown to be useful in obtaining regulated expression from the chromosomal location. In another experiment, it was shown that the integration of a re gulatory gene at the lacA locus was able to control the expression of a tra nscriptional fusion at the amyE locus. These experiments demonstrate that b oth integration sites can be used simultaneously to obtain regulated expres sion of desired genes.