Twelve-transmembrane-segment (TMS) version (Delta TMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity

Authors
Jin, J Guffanti, AA Beck, C Krulwich, TA
Citation
J. Jin et al., Twelve-transmembrane-segment (TMS) version (Delta TMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity, J BACT, 183(8), 2001, pp. 2667-2671
Citations number
28
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
183
Issue
8
Year of publication
2001
Pages
2667 - 2671
Database
ISI
SICI code
0021-9193(200104)183:8<2667:T(V(TV>2.0.ZU;2-K
Abstract
A "Tet(L)-12" version of Tet(L), a tetracycline efflux protein with 14 tran smembrane segments (TMS), was constructed by deletion of two central TMS. T et(L)-12 catalyzed Na+/H+ antiport and antiport with K+ as a coupling ion a s well as or better than wild-type Tet(L) but exhibited no tetracycline-Me2 +/H+ antiport in Escherichia coli vesicles.