Determination of moxifloxacin in growth media by high-performance liquid chromatography

A direct injection high-performance liquid chromatographic method with colu mn switching has been developed to determine moxifloxacin in Mueller-Hinton broth. A LiChrocart 4-4 pre-column filled with a LiChrospher 100 RP 18.5 m um and a 150x4.6 mm I.D, column packed with a Supelcozil ABZ+ Plus were use d and led to a retention time of 5.70 min. Fluorescence detection allowed o ne to reach a quantification limit of 0.05 mug/ml with a 100-mul sample siz e. The standard curves were linear from 0.05 to 3.2 mug/ml. Intra- and inte r-day imprecisions within the linearity range were less than or equal to4.7 6 and less than or equal to5.75%, respectively. The mean relative errors fo r the same day and the day-to-day inaccuracies ranged from -2.93 to +4.50% and from -1.10 to +6.00%, respectively. The method was demonstrated to be u seful for pharmacokinetic-pharmacodynamic studies of moxifloxacin in an in vitro model. (C) 2001 Elsevier Science B.V. All rights reserved.