Optimized analytical method for cyclosporin A by high-performance liquid chromatography-electrospray ionization mass spectrometry

The Micromass Platform LCZ mass detector parameters were optimized for simu ltaneous recording of the protonated (CsA similar toH(+)), sodium adduct (C sA similar to Na+) and potassium adduct (CsA similar toK(+)) of cyclosporin A eluted from a Symmetry Shield RP8 column. The optimized procedure allows a precise analysis of CsA in whole blood or serum without removal of salts prior to analysis. The ratio of the three forms of CsA varied depending on the assay condition and the types of specimens being analyzed. The summati on of three ionic forms of CsA detected by LC-ESI-MS is a reliable and simp le method to assess CsA concentration in the blood. (C) 2001 Elsevier Scien ce B.V. All rights reserved.