Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector

We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of ho mogenate. An internal standard of (ZnS)-S-35 added to the homogenate permit s correction for incomplete recovery of H,S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively respo nds to sulfur-containing compounds, greatly facilitates this measurement. P ublished by Elsevier Science B.V.