Sensitive determination of vinorelbine and its metabolites in human serum using liquid chromatography-electrospray mass spectrometry

Authors
Ragot, S Sauvage, FL Rousseau, A Genet, D Dupuy, JL Tubiana-Mathieu, N Marquet, P
Citation
S. Ragot et al., Sensitive determination of vinorelbine and its metabolites in human serum using liquid chromatography-electrospray mass spectrometry, J CHROMAT B, 753(2), 2001, pp. 167-178
Citations number
29
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
13872273 → ACNP
Volume
753
Issue
2
Year of publication
2001
Pages
167 - 178
Database
ISI
SICI code
1387-2273(20010405)753:2<167:SDOVAI>2.0.ZU;2-D
Abstract
A liquid chromatography-electrospray mass spectrometry method was developed for the quantitation of vinorelbine (VNB) and two metabolites, vinorelbine N-oxide (VNO) and deacetyl vinorelbine (DAV) in human serum. The limits of quantitation (LOQ) reached 0.5 ng/ml for both VNB and VNO and 1 ng/ml for DAV. The method was proved linear in the range of LOQs up to 1000 ng/ml, an d extraction recovery was 80% on average for the three compounds. It was ap plied to the pharmacokinetic monitoring of vinorelbine and, for the first t ime, to the detection of VNO in the serum of patients suffering from non-sm all-cell lung cancer. (C) 2001 Elsevier Science B.V. All rights reserved.