Simultaneous determination of byak-angelicin and oxypeucedanin hydrate in rat plasma by column-switching high-performance liquid chromatography with ultraviolet detection

A simple and sensitive column-switching HPLC method was developed for the s imultaneous determination of two furocoumarin compounds, byak-angelicin and oxypeucedanin hydrate, which are the main components of hot water extract of Angelica dahurica root (AE), in rat plasma. Plasma sample was simply dep roteinated with perchloric acid. After centrifugation, the supernatant was injected into a column-switching HPLC system consisting of a clean-up colum n (Symmetry Shield RP 8, 20x3.9 mm I.D.) and analytical column (Symmetry C- 18, 75x4.6 mm I.D.) which were connected with a six-port switching valve. T he how-rate of the mobile phase (acetonitrile-water, 20:80) was maintained at 1 ml/min. Detection was carried out at wavelength 260 nm with a UV detec tor. The column temperature was maintained at 40 degreesC. The calibration curves of byak-angelicin and oxypeucedanin hydrate were linear over the ran ges 19.6 to 980 ng/ml (r(2)>0.997). The accuracy of these analytes was less than 4.4%. The intra- and inter-day relative standard deviations of byak-a ngelicin and oxypeucedanin hydrate were within 12.0% and 12.7%, respectivel y. The present method was applied for the analysis of plasma concentration from mts after administration of AE. (C) 2001 Elsevier Science B.V. All rig hts reserved.