Determination of lysophosphatidic acids by capillary electrophoresis with indirect ultraviolet detection

Lysophosphatidic acid (LPA) is the simplest form of lysophospholipid. Molec ular species of LPA have been identified as the potent components in the ov arian cancer activation factor. The elevated plasma LPAs may be used as pot ential biomarkers for the early detection of ovarian cancer. This paper is the first report on the quantitative analysis of molecular species of LPA u sing capillary electrophoresis. In this work, the separation of LPAs was ac hieved within 14 min in an adenosine monophosphate-borate-methanol-water so lution, and the measurement was accomplished by indirect UV detection. With LPA (D) as internal standard, the method had linear calibration ranges for LPAs from 2.8 to 75 muM. The detection limits fur various molecular specie s of LPA were from 1.2 to 2.3 muM by the pressure injection at 3.45 kPa for 5 a. The method had been applied to serum fortified with LPA (S), LPA (O), LPA (P), and LPA (M) and the recoveries ranged from 83 to 112%. (C) 2001 E lsevier Science B.V. All rights reserved.