Acute activation of gp130 gene expression in bone marrow stromal cells by contact with myeloma-derived lymphoblastic cell line ARH77 cell membranes

Cell-cell contact of myeloma-derived cell lines (MDCL) or fresh myeloma cel ls with bone marrow stromal cells (BMSC) is known to induce interleukin-6 ( IL-6) and matrix metalloproteinase-1 (MMP-1) production by a marrow stromal cell line, To determine if other BMSC transcripts are altered during cell- cell contact between BMSC and tumor cells, we have used cell lines ARH77 an d U266 in an in vitro model, Using mRNA differential display and reverse tr anscriptase-polymerase chain reaction (RT-PCR), it was determined that a to tal of 141 transcripts were either upregulated or downregulated in the BMSC on contact with cell membrane from cell lines ARH77 and U266, Induction of two of these transcripts, interleukin-6 (IL-6) and gp130 in the BMSC by AR H77 cell membranes was studied in greater detail, Real-time PCR was used to quantitate transcript levels of gp130, IL-6, and 36b4, a housekeeping gene . Cycloheximide (CHX) alone increased both gp130 and IL-6 transcripts in th e BMSC, In addition, CHX caused a superinduction of these transcripts in BM SC exposed to ARH77 cell membranes, The induction of gp130 was independent of the increase in IL-6 mRNA, Upregulation of gp130, a component of the mem brane receptors for the IL-6 superfamily, can have profound effects on the response of BMSC to the IL-6 superfamily of cytokines.