N-(BETA-CARBOXYPROPIONYL) LUMINOL AS A NEW CHEMILUMINESCENCE LABEL INIMMUNOASSAY

Authors
ZHUANG HS WANG QE ZHANG F HUANG QJ TANG YC
Citation
Hs. Zhuang et al., N-(BETA-CARBOXYPROPIONYL) LUMINOL AS A NEW CHEMILUMINESCENCE LABEL INIMMUNOASSAY, Chinese journal of chemistry, 15(2), 1997, pp. 123-129
Citations number
7
Categorie Soggetti
Chemistry
Journal title
Chinese journal of chemistry → ACNP
ISSN journal
1001604X
Volume
15
Issue
2
Year of publication
1997
Pages
123 - 129
Database
ISI
SICI code
1001-604X(1997)15:2<123:NLAANC>2.0.ZU;2-V
Abstract
A new chemiluminescence label N-(beta-carboxypropionyl)luminol (CPL) w as used to label sheep anti-human IgG (SaHIgG). The labeled antibody w as stable and could be detected at least down to 10-(17)similar to 10( -16) mol. The molar incorporation ratio was estimated to be 0.26 mol o f CPL per mol of SaHIgG. There were no apparent changes in the immunor eactivity of the labeled SaHIgG and in the quantum efficiency of the C PL after labeling.