V. Midy et al., Adsorption of vascular endothelial growth factor to two different apatiticmaterials and its release, J MAT S-M M, 12(4), 2001, pp. 293-298
Citations number
32
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
The aim of our study was to assess the ability of calcium phosphate powders
to serve as growth factor carriers. Vascular endothelial growth factor (VE
GF), in particular, is locally involved in the bone formation process throu
ghout osteoblast differentiation. Two different apatitic substrates were te
sted: hydroxyapatite (HA), widely used as biomaterial, and nanocrystalline
carbonated apatite (CA), which has a composition similar to bone mineral cr
ystals. These materials have been compared for their VEGF adsorption and re
lease properties. The adsorption of the growth factor was higher on CA than
on HA probably due to differences of both the proteins and the powders inv
olved. The specific activity of the VEGF released was also tested to determ
ine the available activity for cells in contact with these materials. Inter
estingly, the bioactivity of the VEGF released from CA quantified on fetal
bovine aortic endothelial cells (FBAE) by evaluating the proliferation acti
vity, exhibited no marked difference compared to native VEGF. Qualitatively
, VEGF adsorbed on CA material induced well-defined collagen type I immunos
taining on osteoblast cells compared to the staining obtained after VEGF ad
sorption on HA. (C) 2001 Kluwer Academic Publishers.