D. Paton et al., DETECTION OF TRANSMISSIBLE GASTROENTERITIS VIRUS BY RT-PCR AND DIFFERENTIATION FROM PORCINE RESPIRATORY CORONAVIRUS, Journal of virological methods, 66(2), 1997, pp. 303-309
Citations number
28
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
An RT-PCR method was developed that amplified genetic material from th
e 5' end of the S protein gene of both transmissible gastroenteritis v
irus (TGEV) and porcine respiratory coronavirus (PRCV), but discrimina
ted between the two by the size of the product generated. A number of
restriction endonuclease enzymes were assessed for recognition of the
amplicons so produced. The assay was shown to detect viral RNA from al
l of the 26 different TGEV and PRCV isolates examined, covering a peri
od from 1946 to 1996. Detection of TGEV in clinical specimens was poss
ible using a spin column method to extract RNA and sensitivity was com
pared to virus isolation and antigen detection ELISA. The method could
provide a means of confirming positive results from immunological scr
eening tests such as FAT and ELISA, reducing the need for virus isolat
ion and convalescent serology. (C) 1997 Elsevier Science B.V.