Validated HPLC method for quantifying permethrin in pharmaceutical formulations

An isocratic HPLC method for permethrin determination in raw material and p harmaceutical presentations as lotion and shampoo has been developed and va lidated following ICH recommendations. Cis and trans-isomers, impurities an d degradation products are well separated. The chromatographic analysis wer e performed on a 4 mum particle C-18 Nova-Pak (Waters, Madrid, Spain) colum n (15 x 0.39 cm) kept in a Biorad column oven at 35 degreesC. Mobile phase consisted of methanol-water (78.22, v/v) at a how rate of 1 ml/min. UV dete ction was performed at 272 nm and peaks were identified with retention time s as compared with standards and confirmed with characteristic spectra usin g the photodiode array detector. (C) 2001 Elsevier Science B.V. All rights reserved.