HPLC determination of adenosine in human synovial fluid

A high-performance liquid chromatographic method has been developed for the quantitative determination of adenosine in human synovial fluid. The metho d is simple, rapid and, overall, selective. No interference with the compon ents of the biological matrix was observed in these chromatographic conditi ons. An ODS (250 x 4.6 mm) 5 mum column was used with an isocratic elution of a phosphate buffer acetonitrile mobile phase. Detection was carried out on a UV detector at 260 nm. Calibration curve was found to be linear in the 0.7-70 mug ml(-1) range. Linear regression analysis of the data demonstrat es the efficacy of the method in terms of precision and accuracy. The preci sion of this method, calculated as the relative standard deviation (RSD) of the recoveries (1.57-2.21%) was, excellent. The limits of quantitation (LO Q) and detection (LOD) were respectively 0.7 and 0.2 mug ml(-1). The Inctho d was applied to some samples of synovial effusion From patients affected b y rheumatoid arthritis. The concentrations of adenosine which were Found we re included in the range of the calibration curve. (C) 2001 Elsevier Scienc e B.V. All rights reserved.