Synthesis and structure-activity relationships of di- and trisaccharide inhibitors for Shiga-like toxin Type 1

The syntheses of galabiose and P-k-trisaccharide analogues in which selecte d hydroxy groups are replaced by O-methyl, amino deoxy, acetamido deoxy, an d carboxyalkyl groups are reported. The ability of these inhibitors to bloc k E. coli verotoxin 1 binding to its mammalian cell-surface receptor are ev aluated by a solid-phase competition assay. The synthesis of a biotinylated glycoconjugate for this assay is described, wherein a P-k-trisaccharide te ther derivative 70 is constructed and covalently attached to bovine serum a lbumin followed by biotinylation. Galabiose derivatives 4 and 5 that contai n a carboxymethyl or carboxyethyl substituent at O-2 of the beta -galactose residue show 15-20-fold activity gains over the methyl glycoside of galabi ose. This enhanced activity is not observed for the corresponding carboxyme thyl-substituted P-k-trisaccharide analogue 13. The inhibition data are rat ionalized with the solved crystal structure for verotoxin 1 complexed with a P-k-trisaccharide analogue and provide insight for the design of dimeric inhibitors that can exploit the unique binding-site distribution of the tox in's B subunit. This discussion provides a further example of the important role played by ordered water molecules in sugar-protein complexes.