Fungal endophytes are thought to play a major role in the ecology of many c
ool-season grasses. However, the frequency of endophyte infection in the wi
ld remains poorly known. populations of wild fescues were tested for system
ic fungal endophyte infection by taking samples from four native or natural
ized species throughout southern Illinois (Festuca arundinacea, F. rubra, F
. obtusa, and F. paradoxa), and one of these species (F. arundinacea) from
native populations in England. The two grasses native to southern Illinois
(F. obtusa and F. paradoxa) were highly infected (mean infection of 91 % an
d 100 %, respectively). The introduced grass F. rubra was uninfected. Festu
ca arundinacea showed variable levels of infection among populations in bot
h southern Illinois and England (71 % and 64 %, respectively). The role of
endophytes in natural systems is contingent upon infection levels that appe
ar to vary widely among these four species. Furthermore, the use of agricul
tural cultivars may differentially affect infection levels of naturalized p
lants.