C. Hasel et al., Differential and mutually exclusive expression of CD95 and CD95 ligand in epithelia of normal pancreas and chronic pancreatitis, LAB INV, 81(3), 2001, pp. 317-326
Acinar regression in chronic pancreatitis may be due to immune attack in pa
renchymal areas neoexpressing HLA-DR molecules. CD4(+)Th1 cytotoxic T cells
induce apoptosis of their targets via oligomerizing CD95 (APO-1/Fas) death
receptors on target cells by their CD95 ligand (CD95L). We determined the
expression of GD95 and CD95L in epithelia of normal and chronically inflame
d pancreatic tissues. We applied RT-PCR and Western blotting for CD95L expr
ession profiles, serial frozen section immunohistochemistry to detect CD95,
CD95L, and HLA-DR molecules, CD3, CD4, CD11c, and S-100 protein (S100p). N
ormal pancreases and chronic pancreatitis contain CD95L message and protein
. Immunohistochemistry revealed a mutually exclusive expression of CD95 and
CD95L. Physiologically, acini were CD95(-)/CD95L(+), ducts were CD95(-)/CD
95L(-), and islets were CD95(-)/CD95L(+). In areas of lymphohistiocytic inf
iltration, mainly consisting of CD3(+)CD4(+) T cells and CD11c(+), CD4(+/-)
, /S100p(+) interstitial dendritic cells, and in areas of initial fibrosis,
acini and ducts were HLA-DR+, acini CD95(-)/CD95L(-), and ducts CD95(+)/CD
95L(-). Islet cells were CD95(-)/CD95L(+) in both conditions. IFN gamma lev
els in protein lysates, as measured by an immunoassay, were significantly h
igher in chronic pancreatitis than in normal pancreas (p < 0.0003). In vitr
o, IFN<gamma> downmodulated CD95L message and protein in ASPC1 and BxPc3 pa
ncreatic carcinoma cells. In conclusion, pancreatic epithelia differentiall
y express CD95 and CD95L in a mutually exclusive manner. In chronic pancrea
titis the CD95(-)/CD95L(+) status is conserved in islet cells even in the v
icinity of lymphohistiocytic infiltrates, whereas it is lost in acini coexp
ressing HLA-DR. As a potential consequence, and possibly triggered by local
release of IFN gamma, CD4(+)Th1 cells may cognately interact with and succ
essfully attack exocrine cells by triggering CD95 on their target without b
eing killed by epithelial, CD95L-mediated, counterattack.