Somatostatin induces migration of acute myeloid leukemia cells via activation of somatostatin receptor subtype 2

Somatostatin, a neuropeptide with multiple activities, exerts its function via G-coupled membrane receptors. Five somatostatin receptor subtypes, sst( 1-5), have been identified. We have recently established that somatostatin acts as a chemoattractant on normal hematopoietic progenitor cells. Here, w e studied the expression of somatostatin receptors (sst) on leukemic cells from 16 AML patients. Using fluorescent somatostatin (Fluo-SS) in flow cyto metry, we found that sst are expressed in variable amounts on primary AML c ells. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis and immunochemistry revealed that only sst subtype 2 is expressed by AML cells . Using a two-chamber in vitro migration essay, we show that AML cells migr ated towards a gradient of octreotide, a stable synthetic analogue of somat ostatin. The degree of migration correlated with the cell surface density o f sst, as measured by Fluo-SS binding. These findings indicate that somatos tatin influences trafficking of AML cells, which may have implications for the distribution of AML cells in the body and for clinical applications of somatostatin and analogues thereof in the context of AML.