Recurrence of hepatitis C after liver transplantation is associated with increased systemic IL-10 levels

BACKGROUND: Recurrence of hepatitis C after liver transplantation is an alm ost universal occurrence. T-cell derived cytokines have an important role i n the development of liver damage associated with chronic hepatitis C, thei r post-transplant levels, however, have not been correlated with histologic recurrence of the disease. Aims: We sought to analyze levels of TNF-alpha, soluble IL-2 receptor, IL-4 and IL-10 at 1 month, 6 months and 1 year after transplantation in 27 pati ents undergoing transplantation for hepatitis C related end-stage liver dis ease. Methods: HCV RNA levels were monitored by a branched-chain DNA signal ampli fication assay. Diagnosis of recurrent hepatitis was based on 1-year protoc ol biopsies and on biopsies performed for liver enzyme elevations. Results: Recurrent hepatitis C was detected in 52% (n=14) of the 27 patient s. HCV RNA levels rose over time in all patients regardless of histologic r ecurrence. TNF-alpha, and IL-4 levels, although elevated, did not show spec ific patterns over time or in correlation with recurrence. Similarly, the e arly elevation followed by a gradual decrease over the first year in the am ount of soluble IL-2 receptor was not related to histologic recurrence. We observed a significant increase in circulating IL-10 levels over the first year in patients with biopsy-proven recurrence, while patients with no sign s of histologic recurrence displayed increased, but steady levels. Conclusions: These results suggest that while these cytokines are associate d with post-transplant recurrence of hepatitis C, their production may be a ltered by additional factors.