Effects of insulin on glucose uptake and leg blood flow in patients with sickle cell disease and normal subjects

The hemodynamic concept of insulin resistance assumes that vasodilatory eff ects of insulin determine glucose uptake. Sickle cell disease (SCD) is char acterized by microangiopathy and microvascular occlusion. Therefore, we hyp othesized that patients with SCD have a reduced insulin-mediated glucose up take. In 8 patients with SCD and 8 matched normal controls, we studied the effects of a 4-hour insulin infusion (50 mU/kg/h) on glucose uptake and leg blood flow (LBF) using the euglycemic clamp technique and venous occlusion plethysmography. Time-control experiments were performed in the same subje cts. Insulin-mediated glucose uptake (M value, mg/kg/min) did not differ be tween patients with SCD and control subjects during the second (6.3 +/- 4.6 and 7.6 +/- 2.6, P = .5), third (7.5 +/- 4.6 and 9.3 +/- 3.4, P = .4) and fourth hour (8.6 +/- 4.7 and 11.0 +/- 2.9, P = .2) of the clamp. At baselin e, LBF was higher in the patients with SCD than in the controls (3.28 +/- 1 .68 and 1.37 +/- 0.47 mL/min/dL, respectively; P = .005). Insulin-induced i ncreases in LBF in patients with SCD and in normal subjects were not differ ent (P = .9). Respectively, 56% and 24% of the changes in glucose uptake co uld be explained from changes in LBF in the course of the insulin infusion in the patients with SCD and controls. We suppose that the comparable insul in sensitivity between both groups is due to a compensatory hemodynamic sta te in SCD characterized by vasodilation and increased flow. Copyright (C) 2 001 by W.B. Saunders Company.