RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene

In this study, the effect of a prior UV irradiation on the removal of cyclo butane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-tr anscribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-ir radiation with a dose of 20 J/m(2) enhances the removal of CPDs induced by a second UV dose of 100 J/m(2) in the TS and the NTS of MFA2 gene except fo r the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad 26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential f or inducible NER. (C) 2001 Elsevier Science B.V. All rights reserved.