Differential protein expression in the fruiting dikaryon and the non-fruiting monokaryon of Flammulina velutipes

Patterns of protein expression in the monokaryons and the derived dikaryon of the basidiomycete Flammulina velutipes were investigated using two-dimen sional polyacrylamide gel electrophoresis and silver staining. Over 300 spo ts were detected on each gel, and 19 proteins were expressed in the dikaryo n before fruiting treatment but not expressed in the monokaryon before frui ting treatment. On the of her hand, 9 proteins were expressed in the monoka ryon specifically. Newly expressed proteins increased after fruiting treatm ent (at 7 d and 14 d), but decreased after fruit bodies were formed (at 21 d). The number of newly expressed proteins after the fruiting treatment was similar in the monokaryon. Proteins expressed characteristically were clas sified into 7 types. The highest number of proteins (13 proteins) were spec ifically expressed in the dikaryon at 14 d when the fruit-bodies were forme d. Twenty-seven proteins were expressed specifically in the fruit body. Fru it body specific proteins Pf1 and Pf3 were sequenced. This revealed Pf1 and Pf3 had amino acid sequences that were similar to each other, as well as a high similarity to the deduced amino acid sequence of the FDS gene isolate d from F. velutipes.