Measurement of lactose in bovine raw milk using oligosaccharide oxidase

Authors
Murata, T Amarume, S Usui, T Ohshima, H Abukawa, K Kanzaki, B Hosono, A
Citation
T. Murata et al., Measurement of lactose in bovine raw milk using oligosaccharide oxidase, NIP NOG K-J, 75(4), 2001, pp. 447-449
Citations number
1
Categorie Soggetti
Agricultural Chemistry
Journal title
NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY
ISSN journal
00021407 → ACNP
Volume
75
Issue
4
Year of publication
2001
Pages
447 - 449
Database
ISI
SICI code
0002-1407(200104)75:4<447:MOLIBR>2.0.ZU;2-A
Abstract
A novel method for measuring lactose in bovine raw milk has been developed. The method is based on measuring H2O2 produced from lactose by oligosaccha ride oxidase through a coupled reaction involving peroxidase with a chromog enic reagent. The assay system gave a linear dose response of lactose in th e range of 25-300 mug/mL in 2-20 min of incubation. Lactose concentration i n diluted bovine raw milk was directly determined with high precision by th is method without any pretreatment. This is a quick and simple procedure an d is suitable for multi-sample assay compared to the conventional Lane-Eyno n method.