Synergistic growth inhibition of prostate cancer cells by 1 alpha,25 Dihydroxyvitamin D-3 and its 19-nor-hexafluoride analogs in combination with either sodium butyrate or trichostatin A
Sf. Rashid et al., Synergistic growth inhibition of prostate cancer cells by 1 alpha,25 Dihydroxyvitamin D-3 and its 19-nor-hexafluoride analogs in combination with either sodium butyrate or trichostatin A, ONCOGENE, 20(15), 2001, pp. 1860-1872
Prostate cancer is a major cause of male cancer death. In vitro and in vivo
data support a role for 1 alpha ,25 Dihydroxyvitamin D-3 (1 alpha ,25(OH)(
2)D-3) in regulating the growth and differentiation of the normal prostate
gland yet prostate cancer cells appear significantly less sensitive to this
action. Vitamin D-3 receptor (VDR) content or mutational status do not cor
relate clearly with the antiproliferative effects of 1 alpha ,25(OH)(2)D-3
and therefore it is unclear why prostate cancer cell lines are significantl
y less sensitive to this action. We hypothesized that the antiproliferative
responses of prostate cancer cells to 1 alpha ,25(OH)(2)D-3 are suppressed
by a process involving histone deacetylation, Sodium butyrate (NaB) and tr
ichostatin A (TSA) are inhibitors of histone deacetylase (HDAC) activity. L
ow doses of NaB or TSA (300 muM and 15 nM respectively), which alone were r
elatively inactive, synergized with 1 alpha ,25(OH)(2)D-3 in liquid and sem
i-solid agar to inhibit the growth of LNCaP, PC-3 and DU-145 prostate cance
r cells. Still greater synergy was observed between vitamin D-3 hexafluorid
e analogs and either NaB or TSA, The mechanism appeared to involve neither
the cyclin-dependent kinase inhibitor, p21((waf1/cip1)) nor cell cycle arre
st, but rather induction of apoptosis, These data suggest that cells dysreg
ulate the normal pro-apoptotic signals of 1 alpha ,25(OH)(2)D-3 during pros
tate cancer development by a mechanism involving histone deacetylation, Com
bination therapy with potent vitamin D-3 analogs and clinically approved HD
AC inhibitors may overcome this lesion and improve the treatment of both an
drogen-dependent and independent prostate cancer.