Genetic variation in mRNA coding sequences of highly conserved genes

Citation
Alma. Ten Asbroek et al., Genetic variation in mRNA coding sequences of highly conserved genes, PHYSIOL GEN, 5(3), 2001, pp. 113-118
Citations number
20
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PHYSIOLOGICAL GENOMICS
ISSN journal
10948341 → ACNP
Volume
5
Issue
3
Year of publication
2001
Pages
113 - 118
Database
ISI
SICI code
1094-8341(20010402)5:3<113:GVIMCS>2.0.ZU;2-P
Abstract
The frequency and distribution of genetic polymorphism in the human genome is a question of major importance. We have studied this in highly conserved genes, which encode crucial functions such as DNA replication, mRNA transc ription, and translation. Evolutionary comparisons suggest that these genes are under particularly strong selective pressure, and their frequency of n ucleotide sequence polymorphism would be expected to represent a minimum es timate for sequence variation throughout the genome. We have analyzed the c omplete coding sequence and the 3'-untranslated region (3'-UTR) of 22 human genes, most of which have homologs in all cellular organisms and all of wh ich are at least 25% amino acid identical to homologs in yeast. Comparisons with similar studies of less conserved human disease genes indicate that 1 ) evolutionarily conserved genes are, on average, less polymorphic than dis ease related genes; 2) the difference in polymorphism levels is attributabl e almost entirely to reduced levels of variation in protein coding sequence s, whereas noncoding sequences have similar levels of polymorphism; and 3) the character of polymorphism, in terms of the spectrum and frequency of mu tational changes, is similar.