The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer

This study was conducted to determine the most suitable method of regenerat ion by comparing two approaches: transfer of anthers (with and without embr yo-like structures) to regeneration conditions after a period of two to fou r weeks on induction medium (= anther-transfer treatment) and transfer of e mbryo-like structures to regeneration conditions after five to eight weeks on induction medium. The early transfer of anthers brought about a signific ant reduction in the number of embryos formed, but nevertheless significant ly improved the frequency of plant regeneration. Combining an optimal date of anther transfer with the early addition of colchicine to the induction m edium (100 mg l(-1) for 1 and 3 days) led to an increase in the number of d oubled haploid regenerants. The results indicate that transferring the anth ers after 28 days and adding 100 mg l(-1) colchicine to the induction mediu m on one day only caused a significant improvement in the ability of green plants to regenerate (7.0 compared to 0.50) as well as in chromosome doubli ng (success index: 4.0 compared to 0.33).