Hormonal and histological studies related to in vitro banana bud formation

Citation
Gr. Zaffari et al., Hormonal and histological studies related to in vitro banana bud formation, PL CELL TIS, 63(3), 2000, pp. 187-192
Citations number
32
Categorie Soggetti
Plant Sciences
Journal title
PLANT CELL TISSUE AND ORGAN CULTURE
ISSN journal
01676857 → ACNP
Volume
63
Issue
3
Year of publication
2000
Pages
187 - 192
Database
ISI
SICI code
0167-6857(2000)63:3<187:HAHSRT>2.0.ZU;2-0
Abstract
Shoot apices of Musa subgroup AAA 'Grande Naine' were used for in vitro cul ture establishment. The endogenous hormone levels and their effects on bud formation were evaluated during a 75-day period. Cytokinins, IAA and ABA we re separated by HPLC and quantified by means of ELISA. Enzymatic degradatio n of IAA was determined by the colorimetric method. Explants were maintaine d on establishment medium for 60 days. The endogenous cytokinins were highe r in the basal portion of the explant. Subculture to proliferation medium ( 65 to 75 days) resulted in a substantial increase of cytokinins in the basa l portion and in a decline in the apical portion. 2iP was the predominant c ytokinin in the tissue. The endogenous level of IAA and the IAA/cytokinin r atio decreased after the 65th day of culture. The level of ABA was reduced from the time of inoculation up to the 75th day of culture. Histological an alysis indicated that buds formed at the leaf base at the 65th day of cultu re.