Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation

Citation
Jp. Majada et al., Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation, PL CELL TIS, 63(3), 2000, pp. 207-214
Citations number
32
Categorie Soggetti
Plant Sciences
Journal title
PLANT CELL TISSUE AND ORGAN CULTURE
ISSN journal
01676857 → ACNP
Volume
63
Issue
3
Year of publication
2000
Pages
207 - 214
Database
ISI
SICI code
0167-6857(2000)63:3<207:IOCVVO>2.0.ZU;2-O
Abstract
Dianthus caryophyllus cv. Nelken was cultured in vitro under different vent ilation rates (0.11, 0.21, 0.68 and 0.86 changes h(-1)). Ventilation modifi ed the anatomical characteristics of shoots and leaves described for plants grown in non-ventilated vessels: the cuticle became thicker, there was a d ecreased cell size and intracellular space size. Also, there were more phot osynthetic and supportive tissues, including thicker cell walls. Increased ventilation promoted in vitro hardening of micropropagated carnation shoots , and pushed the culture-induced phenotype closer to that of ex vitro accli matized plants. Anatomical variability of in vitro-grown plants was demonst rated to be a consequence of ventilation.