Jp. Majada et al., Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation, PL CELL TIS, 63(3), 2000, pp. 207-214
Dianthus caryophyllus cv. Nelken was cultured in vitro under different vent
ilation rates (0.11, 0.21, 0.68 and 0.86 changes h(-1)). Ventilation modifi
ed the anatomical characteristics of shoots and leaves described for plants
grown in non-ventilated vessels: the cuticle became thicker, there was a d
ecreased cell size and intracellular space size. Also, there were more phot
osynthetic and supportive tissues, including thicker cell walls. Increased
ventilation promoted in vitro hardening of micropropagated carnation shoots
, and pushed the culture-induced phenotype closer to that of ex vitro accli
matized plants. Anatomical variability of in vitro-grown plants was demonst
rated to be a consequence of ventilation.