High frequency somatic embryogenesis and plant regeneration in zygotic embryo cultures of Liriope platyphylla Wang et Tang

Mature zygotic embryos of Liriope platyphylla Wang et Tang formed embryogen ic calluses at a frequency of 33% when cultured on Murashige and Skoog (MS) medium supplemented with 4.52 muM 2,4-dichlorophenoxyacetic acid. Upon tra nsfer to half-strength MS basal medium, embryogenic calluses gave rise to n umerous somatic embryos, which then developed into plantlets. Regenerated p lantlets were successfully transferred to potting soil, where they were sub sequently grown to maturity.