F. Nogue et al., Higher levels of cell proliferation rate and cyclin CycD3 expression in the Arabidopsis amp1 mutant., PLANT GR R, 32(2-3), 2000, pp. 275-283
Cytokinins are involved in plant cell proliferation leading to plant growth
and morphogenesis. The amp1 mutant of Arabidopsis has a five-fold higher l
evel of cytokinin than the wild type plant. The mutant has a number of phen
otypes with apparently increased cell proliferation rate similar to those s
een in plants transformed with an ipt gene and having higher cytokinin leve
ls. In order to identify molecular factors responsible for this cytokinin-i
nduced higher cell division rate in the mutant we have examined cyclin gene
s expression levels. While expression of the CycD1 and cycC cyclins is not
altered or is slightly decreased in the mutant, cyclin CycD3, is more highl
y expressed. The level of the CycD3 expression decreases in a "revertant" o
f amp1, meaning that the over-expression of this particular cyclin is linke
d to the phenotype of the amp1 mutant. Thus the expression of a specific G1
cyclin is upregulated in a mutant which has more cytokinin than the wild t
ype.