Electron transport chain defect and inefficient respiration may underlie pulmonary hypertension syndrome (ascites)-associated mitochondrial dysfunction in broilers
D. Cawthon et al., Electron transport chain defect and inefficient respiration may underlie pulmonary hypertension syndrome (ascites)-associated mitochondrial dysfunction in broilers, POULTRY SCI, 80(4), 2001, pp. 474-484
By using a series of chemical inhibitors of mitochondrial respiration, a si
te-specific defect in the electron transport chain was identified in mitoch
ondria obtained from broilers with pulmonary hypertension syndrome (PHS; as
cites). Located at the succinate:ubiquinone oxido-reductase (Complex II:CoQ
) interface, this defect would allow electrons to leak from the respiratory
chain and consume oxygen by forming reactive oxygen species at a greater r
ate than in control mitochondria. Lower levels of the primary antioxidants,
alpha- and beta -tocopherol, and glutathione (GSH) in PHS mitochondria con
firmed the presence of oxidative stress. Respiration studies of PHS Liver m
itochondria also revealed disease-associated decreases in the respiratory c
ontrol ratio (RCR, an index of electron transport chain coupling). Differen
ces in the RCR as well as the adenosine diphosphate (ADP) to O ratio (an in
dex of oxidative phosphorylation) between control and PHS mitochondria were
accentuated by sequential additions of ADP to isolated mitochondria. Ln a
second experiment, similar improvements in functional indices following seq
uential additions of ADP and responses to respiratory chain inhibitors were
observed in liver mitochondria isolated from Single Comb White Leghorn (SC
WL) males (resistant to PHS) similar to that observed in control broiler mi
tochondria in Experiment 1. The combined results indicate the presence of a
site-specific defect at either Complex II, ubiquinone, or both in liver mi
tochondria obtained from broilers with PHS that may be responsible for the
oxidative stress and mitochondrial dysfunction observed in this costly meta
bolic disease.