FAR-FIELD FLUORESCENCE MICROSCOPY WITH 3-DIMENSIONAL RESOLUTION IN THE 100-NM RANGE

Authors
HELL SW SCHRADER M VANDERVOORT HTM
Citation
Sw. Hell et al., FAR-FIELD FLUORESCENCE MICROSCOPY WITH 3-DIMENSIONAL RESOLUTION IN THE 100-NM RANGE, Journal of Microscopy, 187, 1997, pp. 1-7
Citations number
27
Categorie Soggetti
Microscopy
Journal title
Journal of Microscopy → ACNP
ISSN journal
00222720
Volume
187
Year of publication
1997
Part
1
Pages
1 - 7
Database
ISI
SICI code
0022-2720(1997)187:<1:FFMW3R>2.0.ZU;2-G
Abstract
We report three-dimensional (3D) microscopy with nearly isotropic reso lution in the lambda/5-lambda/10 range. Our approach combines 4Pi-conf ocal two-photon fluorescence microscopy with image restoration. The 3D resolution is demonstrated with densely clustered beads as well as wi th F-actin fibers in mouse fibroblast cells. A comparison with unresto red two-photon confocal images reveals a total reduction of the uncert ainty volume up to a factor of 15.