A 3-compartment model of phytoplankton growth dynamics has been coupled wit
h a primitive-equation circulation model to better understand and quantify
physical and biological processes in the Adriatic Sea. This paper presents
the development and application of a data assimilation procedure based on o
ptimal control theory. The aim of the procedure is to identify a set of mod
el coefficient values that ensures the best fit between data and model resu
lts by minimizing a function that measures model and data discrepancies. In
this sense, twin experiments have been successfully implemented in order t
o have a better estimation of biological model parameters and biological in
itial conditions.