C. Landon et al., Magnetization transfer from laser-polarized xenon to protons located in the hydrophobic cavity of the wheat nonspecific lipid transfer protein, PROTEIN SCI, 10(4), 2001, pp. 762-770
Nonspecific lipid transfer protein from wheat is studied by liquid-state NM
R in the presence of xenon. The gas-protein interaction is indicated by the
dependence of the protein proton chemical shifts on the xenon pressure and
formally confirmed by the first observation of magnetization transfer from
laser-polarized xenon to the protein protons. Twenty-six heteronuclear nOe
s have allowed the characterization of four interaction sites inside the wh
eat ns-LTP cavity, Their locations are in agreement with the variations of
the chemical shifts under xenon pressure and with solvation simulations. Th
e richness of the information obtained by the noble gas with a nuclear pola
rization multiplied by similar to 12,000 makes this approach based on dipol
ar cross-relaxation with laser-polarized xenon promising for probing protei
n hydrophobic pockets at ambient pressure.