Production of ligninolytic enzymes by Fusarium solani strains isolated from different substrata

A comparative study on the extracellular ligninolytic enzymatic activity of five strains of Fusarium solani in a carbon-limited medium under shaking, revealed a differential production of these enzymes. Aryl alcohol oxidase ( AAO) activity was observed only in the supernatant of strain CLPS no. 568 w ith levels higher than 57 mU ml(-1) Free extracellular laccase activity was detected in strains CLPS nos. 493, 568 and 570, strain no. 568 being the o ne which showed the highest activity (over 8.6 mU ml(-1)). Free extracellul ar lignin peroxidase (LiP) activity was not detected in any isolate tested, whereas low levels of manganese-dependent peroxidase (MnP) and manganese-i ndependent peroxidase (MIP) activities were detected in certain isolates us ed. The AAO activity of F. solani on primary alpha -alcohols such as veratr yl alcohol, is reported for the first time; this enzyme activity is hydroge n-peroxide independent. This is also the first report for extracellular MnP and MIP activities of F. solani.