Effects of low density lipoprotein and oxidized low density lipoprotein onthe cytotoxic activity of Asp-hemolysin to murine macrophages

We examined the effects of human low density lipoprotein (LDL) and oxidized LDL (Ox-LDL) on the cytotoxic activity of Asp-hemolysin from Aspergillus f umigatus Fresenius-Muramatsu strain to mouse peritoneal macrophages (M phi) . The inhibitory effects of LDL and Ox-LDL on the cytotoxic activity of Asp -hemolysin to M phi increased in a dose-dependent manner, and the effect of Ox-LDL was greater than the inhibitory effect of LDL. Furthermore, the bin ding of Asp-hemolysin to LDL or Ox-LDL was observed by western blot analysi s of the culture medium. These results suggest that the inhibition by LDL o r Ox-LDL on the cytotoxic activity of Asp-hemolysin to M phi was due to the binding of LDL or Ox-LDL to Asp-hemolysin in the culture medium.