ISOLATION OF HEMATOPOIETIC STEM-CELLS FRO M UMBILICAL HUMAN BLOOD FORTRANSPLANTATION

Transplantation of umbilical blood cells finds more and more wider cli nical application. Practically promising are design of techniques prov iding isolation and enrichment of hemopoietic stem cells for creation of umbilical blood banks and assessment of such techniques efficacy. S eparation with the use of percol/ficol and immunomagnetic selection of CD34(+) cells is highly effective and in case of CD34(+) fraction is associated with 94-fold enrichment of cell-precursors. This fraction i s capable of multiple elevation of cell-precursors count in long-term liquid culture with addition of growth factors. Both fractions secure long-term hemopoiesis on stromal sublayer to the degree proportional t o that of enrichment with hemopoietic stem cells. Safety of whole umbi lical blood is maintained at 22 degrees C or 4 degrees C for 3 days.