Adrenergic stimulation of rat resistance arteries affects Ca2+ sparks, Ca2+ waves, and Ca2+ oscillations

Confocal laser scanning microscopy and fluo 4 were used to visualize local and whole cell Ca2+ transients within individual smooth muscle cells (SMC) of intact, pressurized rat mesenteric small arteries during activation of a lpha (1)-adrenoceptors. A method was developed to record the Ca2+ transient s within individual SMC during the changes in arterial diameter. Three dist inct types of "Ca2+ signals" were influenced by adrenergic activation (agon ist: phenylephrine). First, asynchronous Ca2+ transients were elicited by l ow levels of adrenergic stimulation. These propagated from a point of origi n and then filled the cell. Second, synchronous, spatially uniform Ca2+ tra nsients, not reported previously, occurred at higher levels of adrenergic s timulation and continued for long periods during oscillatory vasomotion. Fi nally, Ca2+ sparks slowly decreased in frequency of occurrence during expos ure to adrenergic agonists. Thus adrenergic activation causes a decrease in the frequency of Ca2+ sparks and an increase in the frequency of asynchron ous wavelike Ca2+ transients, both of which should tend to decrease arteria l diameter. Oscillatory vasomotion is associated with spatially uniform syn chronous oscillations of cellular [Ca2+] and may have a different mechanism than the asynchronous, propagating Ca2+ transients.