Glucagon-like peptide-1(7-34) amide stimulates surfactant secretion in human type II pneumocytes

To determine the influence of glucagon-like peptides on the secretion of hu man pulmonary surfactant, we used human type II pneumocytes. In these cells , CLP-1(7-36) amide and exendin-4 stimulated phosphatidylcholine secretion (PC) and cAMP formation in a concentration-dependent manner; these effects were reversed by exendin(9-39). No changes were observed with other related peptides. The mechanism by which CLP-1(7-36) amide exerts its stimulatory effect was investigated with various agents that are well known to be stimu lators or inhibitors of PC secretion. Thus, 8-bromo-cAMP increased and both Rp-cAMPS and H-89, the latter an inhibitor of protein kinase A (PKA), redu ced pulmonary surfactant secretion in type II pneumocytes. Also, CLP-1(7-36 ) amide and TPA exerted additive effects in stimulating PC secretion, and C alph C, a potent inhibitor of protein kinase C (PKC), blocked most of the e ffect of CLP-1(7-36) amide. By contrast, both the calcium ionophore A23187 and GLP-1(7-36) amide had additive effects in increasing PC secretion, and the specific inhibitor of Ca2+-calmodulin-dependent protein kinase (Ca-CM-P K), KN-62, inhibited the effect of A23187 but did not alter the stimulatory action of GLP-1(7-36) amide. Our findings suggest that both PKA and PKC ar e involved in the stimulatory effects of GLP-1(7-36) amide on PC secretion, whereas this peptide has no effect on PC secretion through a Ca-CM-PK mech anism.