In this article we review tandem mass spectral approaches to the structural
characterization of flavonoid glycosides which are ubiquitous secondary pl
ant metabolites. It is demonstrated how structural information on O-diglyco
sides can be obtained on (i) the carbohydrate sequence, (ii) the aglycone p
art, and (iii) the linkage in the diglycosidic part. Both the positive and
negative ion modes have been employed.