New Fourier transform infrared based computational method for peptide secondary structure determination. II. Application to study of peptide fragments reproducing processing site of ocytocin-neurophysin precursor
M. Simonetti et C. Di Bello, New Fourier transform infrared based computational method for peptide secondary structure determination. II. Application to study of peptide fragments reproducing processing site of ocytocin-neurophysin precursor, BIOPOLYMERS, 62(2), 2001, pp. 109-121
A new method for the quantitative determination of the percentage of intram
olecular H-bonds, based on Fourier transform infrared techniques, is applie
d to the conformational analysis of a series of synthetic peptides spanning
the processing site of the ocytocin and neurophysin precursor. Even though
the method uses traditional tools such as Fourier self-deconvolution, the
Nth derivative, and curve-fitting procedures for the analysis of the spectr
a, the assignment of the absorptions due to peptide groups participating in
to secondary structures is based on the direct observation and quantificati
on of the isotopic effect induced on the groups participating in intramolec
ular H-bonds in the presence of organic solvents. This permits the quantifi
cation of the different populations of molecules containing intramolecular
H-bonds involved in p-turns and cu-helices. The results are consistent with
those previously obtained by NMR techniques in the same solvent systems. (
C) 2001 John Wiley & Sons, Inc.