Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting

Authors
Takai, T Akagawa-Chihara, M Yokota, T Okumura, Y
Citation
T. Takai et al., Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting, BIOS BIOT B, 65(3), 2001, pp. 694-697
Citations number
23
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
65
Issue
3
Year of publication
2001
Pages
694 - 697
Database
ISI
SICI code
0916-8451(200103)65:3<694:ROMOAM>2.0.ZU;2-#
Abstract
A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, sis cysteine mutants, six proline mutants, and three lysine mutants, mere expressed in Escherichia coli, The cells we re solubilized and run on sos-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis.