The est1 regulation depends on the oxygen concentration in Acetobacter pasteurinanus

The regulation mechanism for expression of the ethanol inducible esterase g ene, est1, was investigated in A. pasteurianus. Deletion analysis of the 5 ' non coding region of est1 showed that the FNR-binding consensus sequence is important in the induction of est1 by ethanol. Cells grown under oxygen starvation produced esterase-l in not only the presence but also the absenc e of ethanol. These results suggest that the induction of est1-expression d epends on the oxygen concentration, and the gene may be induced by a FNR-li ke factor activated by a decrease in the intracellular oxygen concentration .