The chemical and nutritional properties were investigated of hypoallergenic
wheat flour (HWF) prepared by the cellulase-actinase treatment, HWF was co
mposed mainly of oligopeptides and free amino acids, and its average molecu
lar weight was lower than 1,000, Feeding tests on rats showed that, with re
spect to the PER, GOT and GPT activities and other nutritional indices, the
NIT;F diet was almost equivalent to the control diet which had been prepar
ed from normal wheat flour (NWF). No abnormality was apparent in the main o
rgans after the HWF diet had been fed for 3 weeks. The small intestinal abs
orption of the HWF diet was found normal by measuring the free amino acid c
oncentration in the intestinal tract and in the portal vein plasma. These d
ata suggest that the absorption of amino acids from the HWF diet was compar
able with or more efficient than that from a simulated free amino acid diet
.