Production and purification of recombinant Blomia tropicalis group 5 allergen from Pichia pastoris culture

Authors
Goh, LT Kuo, IC Luo, S Chua, KY White, M
Citation
Lt. Goh et al., Production and purification of recombinant Blomia tropicalis group 5 allergen from Pichia pastoris culture, BIOTECH LET, 23(9), 2001, pp. 661-665
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
9
Year of publication
2001
Pages
661 - 665
Database
ISI
SICI code
0141-5492(200105)23:9<661:PAPORB>2.0.ZU;2-S
Abstract
The gene of Blomia tropicalis group 5 allergen (Blo t 5) was cloned and exp ressed in Pichia pastoris KM71. Selected KM71 clones were cultivated in a f ed-batch bioreactor feeding first glycerol then followed by methanol. Recom binant Blo t 5 constituted about 30% of the total broth protein after 60 h cultivation. The harvested broth was purified to > 95% purity by a two-step anion exchange chromatography. The overall yield was 37 mg Blo t 5 per lit re broth.