2,5-Diketo-D-gluconate production by a mixed culture of two newly-isolatedstrains: Flavimonas oryzihabitans and Pseudomonas cepacia

Authors
Sulo, P Hudecova, D Properova, A Basnak, I Sedlacek, I
Citation
P. Sulo et al., 2,5-Diketo-D-gluconate production by a mixed culture of two newly-isolatedstrains: Flavimonas oryzihabitans and Pseudomonas cepacia, BIOTECH LET, 23(9), 2001, pp. 693-696
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
9
Year of publication
2001
Pages
693 - 696
Database
ISI
SICI code
0141-5492(200105)23:9<693:2PBAMC>2.0.ZU;2-G
Abstract
A mixed culture of two Gram-negative bacteria isolated from soil converted 50 g D-glucose l(-1) to 2,5-diketo-D-gluconate (2,5 DKG) in 92% yield withi n 150 h. The first strain, producing 2-keto-D-gluconate (2 KDG) from d-gluc ose via d-gluconate (DG), was classified as Flavimonas oryzihabitans. The s econd strain, that converts 2 KDG to 2,5 DKG, was identified as Pseudomonas cepacia. This approach presents a new possibility to produce ascorbic acid by microbial transformation, including the use of other, more convenient s ubstrates.