Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria

Authors
Shuhaimi, M Ali, AM Saleh, NM Yazid, AM
Citation
M. Shuhaimi et al., Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria, BIOTECH LET, 23(9), 2001, pp. 731-736
Citations number
21
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
9
Year of publication
2001
Pages
731 - 736
Database
ISI
SICI code
0141-5492(200105)23:9<731:UOERIC>2.0.ZU;2-Y
Abstract
Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic b acteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that al l of the probiotic bacteria tested possess enterobacterial repetitive inter genic consensus sequences with the PCR products ranging from 250 bp to 5000 bp. The bacterial strains can be differentiated by comparing fingerprint p atterns. The dendrogram of the fingerprints revealed that most of the bifid obacterial wild type strains fell into one cluster at similarity level of a pproximately 79%.