B. Junker et al., Scale-up studies on a defined medium process for pilot plant production ofillicicolin by Gliocladium roseum, BIOTECH PR, 17(2), 2001, pp. 278-286
Illicicolin was cultivated at the 600-L pilot scale for purposes of materia
l generation and process development. The initial medium containing oat flo
ur was difficult operationally as a result of excessive foaming during ster
ilization, so a new defined medium process (with either glucose or sucrose
as the carbon source), developed at the 23-L scale, was scaled up and impro
ved for pilot scale needs. Pilot scale media development efforts focused on
exploring the highest concentration of media (1.0 x to 3.0x) that could be
cultivated at the pilot scale and not be limited by mixing or oxygen mass
transfer. The process was scaled up successfully and peak titers improved 7
.5-fold, from about 200 mg/L in the initial complex medium to 1500 mg/L in
the final defined medium.