Optimization of fermentation conditions for preparation of polygalacturonic acid transeliminase by Erwinia carotovora IFO3830

paper is concerned with optimization of the fermentation conditions for pre paration of alkaline pectin lyase (poly-galacturonic acid trans-eliminase, FATE) by Erwinia carotovora IFO3830 (E.C.IFO3830). The orthogonal matrix me thod was adopted as the experimental design method for media. The effects o f media composition on the growth rate of E.C.IFO3830 are in the order of p ectin > KH2PO4/Na2HPO4 > MgSO4 > (NH4)(2)SO4 > glucose > L-glutamate sodium , and those on FATE activity are in the order of glucose > pectin > L-gluta mate sodium > KH2PO4/Na2HPO4 > MgSO4 > (NH4)(2)SO4 The strain of E.C.IFO383 0 grows quickly and easily near an initial pH of 7.0, and especially at the alkaline range of pH 7.0-8.0. The growth rate profiles indicate that E.C.I FO3830 grows very quickly; its generation time t(1/2) is about 0.2 h. The a ppropriate fermentation period is about 10-20 h for a high level productivi ty of biomass and 25-35 h for high productivity of FATE enzyme.